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Nevertheless, our thresholds were on the higher side to improve stringency.
To our knowledge, this is the first description of exogenous blocking DNA being used to improve stringency of MBD-based assays.
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Therefore, to reach improved stringency in CNV calling and to utilize methods implemented in both ExomeCopy and ExomeDepth, we designed an algorithm (ExCopyDepth) combining these two programs (Flow and implementation of ExCopyDepth; Additional file 1: Figure S2).
The retrobiosynthetic concept was designed to improve the stringency of isotope incorporation studies.
To further improve selection stringency, a series of weakened SV40E promoters have been generated and used to modulate plasmid-based GS expression with the intent of manipulating GS-CHO selection, finely adjusting the balance between GS expression and GS inhibitor (MSX) levels.
To improve the stringency of our analysis we considered genes as changed only if they had a t-test and a TNoM p-value <0.05 and a fold ratio >2 as previously described [20].
Another expression vector engineering approach is to improve selection stringency [ 31].
To improve the stringency, we included only mouse database from IPA for the network/pathway analysis.
Schwartz et al. have proposed some guidelines to improve the stringency of the Then-test method [ 41].
To further improve the stringency, we applied one-way ANOVA analysis on the differentially expressed genes identified by SAM.
In this study, in order to improve the stringency of detecting PABC and to avoid coincident cases, we restricted our sampling to tumours occurring up to 6 months after delivery.
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