Sentence examples for improve sequencing efficiency from inspiring English sources
The KAPA Stranded RNA-Seq Kit with RiboErase uses a targeted enzymatic depletion method to remove ribosomal RNA (rRNA) and thereby improve sequencing efficiency.
Development of a computational methodology may improve sequencing efficiency with this method, through optimisation of 5-methyl-dCTP concentration and prediction of coverage for each nucleotide.
Overall, these results indicate the potential for high-throughput sequencing based on the 454-GS FLX Titanium to improve sequencing efficiency in future transcriptome analyses.
In summary, post-amplification normalization improves sequencing efficiency and genome assembly, and in its absence, bioinformatic normalization may be required for de novo assembly.
Moreover, the paired-end sequencing strategy of RNA-seq further improves sequencing efficiency and extends short read lengths for better understanding transcriptome [ 16].
Overall, these comparisons with previous studies highlight the potential for our method to improve sequencing depth and efficiency in future transcriptome sequencing efforts.
Recently developed next-generation sequencing (NGS) technologies significantly improve the sequencing efficiency and cost.
The latest paired-end tag sequencing strategy of RNA-seq further improves the DNA sequencing efficiency and expands short-read lengths, providing a better depiction of transcriptomes [ 21].
131, 132 Interestingly, by changing the start codon AUG to GCU, improved IRES sequence efficiency was reached in CHO lysate.
In this study it was found that transfection efficiency and the length of homologous sequences improve the efficiency of recombination in these methods, but the targeting of the locus to be engineered by CRISPR/Cas9 nucleases has an overriding positive impact.
The emerging interest in characterizing the effects of members of the rare biosphere in a range of clinical and environmental contexts, combined with the rapid decrease in sequencing cost, challenges us to improve the efficiency of sequence analysis so that that computational cost does not become a limiting factor.
