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To increase sensitivity and improve sequence coverages of ASFV proteins in the mass spectrometric analysis, two replicates of each of the three infected cell lines were digested with three different proteases with complementary specificity (trypsin, chymotrypsin and Glu-C).
This new representation of 3-dimensional features in protein motifs, such as those arising from structural or functional constraints on the sequence, can be used to improve sequence analysis tools including pattern analysis and database search.
For example, Mann et al. [18] presented a semi-supervised CRF to improve sequence segmentation and labelling.
Standard and long range PCR was used to close gaps, improve sequence quality and resolve any remaining base-conflicts.
To improve sequence coverage, we used a size selection procedure that removed cDNAs shorter than 1.3 kb in length.
Additional directed sequencing was performed as necessary to improve sequence quality genome-wide to a minimum base call error rate of 1 in 10,000 (i.e. Q40).
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Please join Professor Yanyi Huang as he discusses his team's newly developed error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy.
The KAPA Stranded RNA-Seq Kit with RiboErase uses a targeted enzymatic depletion method to remove ribosomal RNA (rRNA) and thereby improve sequencing efficiency.
We have incorporated these observations into an N-glycosylation prediction tool which combines structure- and sequence-based rules that significantly improve sequence-based prediction methods.
The next-generation sequencing (NGS) technologies improve sequencing depth and render large-scale EST projects more feasible [ 25- 27].
In addition, the reverse primer HrU10-EXT-R2 (5'-GCTGCTGTTCGAGGAAATAA-3') was designed to improve sequencing of tree swallow alleles.
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