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For optimal evaluation of double staining, we used spectral imaging and image analysis software (Nuance spectral imaging system, CRi; Woburn) to improve perceptibility of each individual color in immune double stains.
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The frequency components of the signal with power levels that fall below the absolute threshold of hearing (ATH) can be discarded, as they do not contribute in improving perceptibility of the signal.
When larger stimuli were used to improve stimulus perceptibility and an eye-tracker monitored fixation accuracy (Experiment 2), left and right increases in the number of letters again produced near-identical effects on reaction time (and accuracy), but frequent and substantial fixation errors were revealed.
Embedding the watermark in high frequency components improves the perceptibility of the watermarked image.
Since the human visual system is less sensitive to the edges, embedding the watermark in the directional subband improves the perceptibility of the watermarked image, but it is hardly robust.
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