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Addition of a sorbitol co-feed has been previously shown to improve overall yields of proteins expressed in Pichia during methanol induction.
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As suggested by Table 3, longer incubation time is likely to further improve overall yield of the desired RNA fragment.
In the first protocol modification, several library preparation steps were optimized including efficiency of adaptor ligation [ 9], but this protocol did not improve overall yield significantly enough to reduce the amount of input DNA.
We believe that the results presented here will assist in improved implementation of DNA tile based algorithmic self-assembly, DNA based molecular robotics, and other specific nucleic acid systems, and will provide guidance to design and assembly processes to improve overall yield and efficiency.
The synthesis was accomplished using an Fmoc-based strategy along with C-terminal labeling for improved overall yield.
Application of a high rate of fertiliser improved overall yield and P offtake further at summit and slope positions, but not at the foot position.
Modification of translation initiation regions (TIRs) to reduce protein translation rates has also had some success at improving overall yield [12].
Also along these lines, methods to increase the leaf:stem ratio would improve overall glucose yields from combined pretreatment and enzymatic hydrolysis.
Prehydrolysis has been shown to reduce the viscosity of the material and also improve overall ethanol yield [ 27].
Therefore, deacetylation of the feedstock before low-severity pretreatment by alkaline extraction could potentially improve overall ethanol yield compared to standard high severity pretreatment techniques.
Switching glucose transport from the phosphoenolpyruvate:sugar phosphotransferase system (PTS) to proton symport has been shown to improve overall performance and production yield for ethanol as well as other compounds [ 35].
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