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To prevent the accumulation of mis-translated proteins arising from ribosome slippage, improper translation initiation or sequence errors, we introduced "ambush" stop codons in the 2nd and 3rd reading frames approximately every 100 base pairs by substitution of synonymous codons.
Even with a correct primary sequence, an improper translation elongation rate in a heterologous expression system is an important cause of misfolding.
The paucity of chimeric TE insertions in exons relative to introns demonstrates that the deleterious effects of chimeric TE insertions must exceed the cost of simply being transcribed, and probably results from improper translation or disruption of other functions of the mRNA such as localization or stability.
Similar(55)
Cryptic Shine-Dalgarno sequences upstream from any AUG in all three reading frames were also removed to prevent improper cryptic translation initiation.
Since there are several stop codons and polyadenylation signals in the 5'-UTR of 380 nucleotides of the major TnT cDNA, it seems that the shorter N terminal in the tarantula major TnT is not due to improper conceptual translation.
In these cases, the translators were asked to compare their translations with the original words from the list and decide whether they would translate the words this way or if they found the Polish translation improper.
This study indicated that an insufficient cultural specificity had resulted in improper and disconnected knowledge translation (54).
First, by indirectly downregulating 14-3-3 14-3-3 14-3-3 leads to the improper mitotic cap-independent translation of important executioners of mitosis, cytokinesis failure and accumulation of binucleate tetraploid cells, and is frequently seen in many cancers, including breast, prostate and BCCs.
Having considered the theological arguments on both sides, the (perfectly secular) court agreed that the translation was "illegal, improper and incorrect" and in violation of Catholic canon law.To anyone from America, France or even semi-theocratic England, it would seem astonishing that state judges would presume to delve into such metaphysical matters.
This may be due to an improper folding of in vitro translated Woc or a requirement for specific posttranslational modifications that do not occur during in vitro translation and bacterial expression.
This on-the-spot translation may have resulted in improper or inaccurate interpretations of the questions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com