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If one selects an appropriate template but produces an improper sequence alignment during the homology-modeling, the final structure may have extended segments in wrong places, but a good part of the 3D-model will still have a low RMSD from the template.
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(b) Multiple sequence alignment of NucS sequences.
Figure 5: Multiple sequence alignment.
Sequence alignment of Piezo orthologues.
R.V.C. contributed the sequence alignment.
b, Sequence alignment of TcB sequences.
c, Sequence alignment of TcB sequences.
Multiple sequence alignment was performed by Muscle alignment using MEGA740.
Multiple sequence alignment of human PADs.
(a) Sequence alignment of TomB/YmoB proteins.
Multiple sequence alignment of gasdermin family members.
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