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The rates of improper segregation and abnormal polar bodies between the control (with control siRNA injection) and RNAi groups are presented in Fig. 6B.
To confirm that the oocytes with improper segregation in Fig. 6Ag-i did enter AI, another group of RNAi oocytes collected at the same time point and under the same culture conditions as those in Fig. 6Ag-i were subjected to Western blot to analyze the cyclin B level.
Additionally, the mitotic instability indicates improper segregation of B chromosomes during mitosis.
Most aneuploidies result from improper segregation of the chromosome pairs during meiosis.
The improper segregation of said proteins in turn leads to unrestricted neuroblast division and outgrowth.
Several studies have demonstrated that improper mitotic spindle orientation causes improper segregation of proteins that are normally asymmetrically localized.
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Additionally, we found that Bub3-RNAi caused misalignment of chromosomes, improper segregations, or abnormal polar bodies (Fig. 6A and B), which was similar to that in Mad2-depleted oocytes exhibiting large or multiple polar bodies [64].
The high expression of Ki67 and Bcl-2 in combination with the non-functional p53 could have contributed to cell tumourigenicity, regrowth of tumour cells, and the formation of radiation-damaged multinucleated giant cells with improper chromosome segregation and cell division behaviours [41, 45, 46].
In mitosis, improper chromosome segregation often leads to cell death through mitotic catastrophe [1] and has also been linked to cancer through formation of aneuploid cells [2], [3].
Mutations in central components of the RNAi machinery result in improper chromosome segregation [ 74].
The presence of multiple poles in GF treated cells probably results in improper chromosome segregation leading to the formation of multiple nuclei.
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