Sentence examples for impossible to successfully amplify from inspiring English sources

But due to the low copy number (usually only one copy per genome) of every exon and the extremely high complexity of the genome, it is usually impossible to successfully amplify the full-length cDNA sequence by overlapping primers located at the precise splicing sites which are not possible for software optimization.

The procedure has been employed to successfully amplify the coding sequence of 10 human cancer genes in one assay with uniform abundance of the final products.

The method was used to successfully amplify three GL-7-ACA acylase genes from different soil samples.

The study conducted by Wegner et al. (2009) was not able to successfully amplify autosomal fetal alleles from maternal plasma.

To our knowledge, this is the first protocol to successfully amplify Avipoxvirus from the blood of naturally infected, wild passerines.

The matK along with the reported matK3.2r primer was able to successfully amplify in Berberis, Ficus and Gossypium.

However, we were able to successfully amplify the 571 bp long TSC2 promoter region.

The developed primers were found to successfully amplify 14 microsatellite loci in P. maritima.

Bybee et al. (2011) used our TAS approach to successfully amplify and sequence DNA from multiple museum specimens.

In most of the cases a nested PCR protocol was applied to successfully amplify the targeted loci.

In the present study, a PCR-based strategy with primers designed on exons was used to successfully amplify NPR1 exons.