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Exact(11)
Until now many researchers have argued that it would be impossible to sequence the entire genome of an extinct creature because the samples are so degraded.
However, because of the effects of later upheavals, it is almost impossible to sequence these early rock layers correctly.
Second, it is essentially impossible to sequence and assemble all nucleotides in the genome (Ellegren 2014).
Before multiple parallel sequencing was developed, it was impossible to sequence multiple times the genome of the same patient.
In some cases, the underlying mutations can not be found at all, because it is impossible to sequence complete genes routinely.
The same H. acutifolia sample that was successful for this study previously proved impossible to sequence for low-copy markers using a Sanger protocol (Folk and Freudenstein, 2014; similar observations in Cronn et al., 2012).
Similar(48)
Before it is over, Pam has delivered her baby, abandons it temporarily in a fit of pique, and in an extended, nearly impossible to watch sequence in which Fred's gang collectively succumbs to the lure of mindless blood lust, the baby is horribly tortured and finally stoned to death in its carriage.
Such DNA poses two problems: The repeats can spontaneously multiply or shrink in the cell, and it's almost impossible to accurately sequence them.
The abundance of repeated motifs with very high similarity and rearrangements in clones made it impossible to completely sequence and assemble BACs 10c2 and 67l12.
Because LNA® probes are much shorter they might be especially appropriate when high specificity is required and where the design of a common TaqMan® probe is difficult or even impossible due to sequence characteristics.
In addition to the fact that humans are diploid, with cells harboring two genomes from the parents, the presence of numerous repetitive elements that are longer than the usual sequencing library insert size makes it close to impossible to assemble genome sequences with short-read sequencing alone (El-Metwally et al. 2013).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com