Sentence examples for impossible to amplify from inspiring English sources

But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the genome by ordinary PCR based techniques.

Since polyploids contain multiple distinct copies of each nuclear gene, known as homeologues, it is usually impossible to amplify a homogeneous, single amplicon using PCR.

Proteobacteria appeared to be less abundant in high emitters, but DNA from one sample proved impossible to amplify to produce an acceptable melt curve.

But due to the low copy number (usually only one copy per genome) of every exon and the extremely high complexity of the genome, it is usually impossible to successfully amplify the full-length cDNA sequence by overlapping primers located at the precise splicing sites which are not possible for software optimization.

Unfortunately, louse data from the remaining 7 families could not be obtained due to specimen rarity (some anopluran families have extremely narrow host ranges and some are monotypic, making collection almost impossible) or failure to amplify specimens in the laboratory.

Although every attempt was made to amplify sequences from identical tissues from all patients, this was impossible due to several factors, including the fact that many brain tissues, especially from those patients without extensive brain disease, contained no amplifiable HIV.

Thus it was impossible to distinguish suitable primers pairs flanking L1 inserts that were homozygous present in HeLa DNA from an unsuccessful reaction (failure to amplify an existing empty site).

We partner with artists to amplify it.

Then they used enzymes to amplify them.

He loves to itemise, to catalogue, to amplify.

DNA was amplified by PCR using primer pairs designed to amplify TFPI2 promoter or TET1 promoter.