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Fully expanded leaves were sampled immediately after the imposition of heat stress treatment (0 h) and after 1, 4 and 8 h of exposure to 42°C.
It is possible that the increased physiological strain observed in the STHA group, and not the imposition of heat per se, drove the cross acclimatory affect [ 46].
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Aside from the imposition of periodic heat-shock, the selection experiment was designed to be highly similar to the general culture conditions that the founding clones previously experienced during their 300 generations of evolution under low versus high co-infection (cf. Figures 1 and 5).
Many large manufacturers and the majority of the big utilities support it, for now, as the least-bad option for what is considered the inevitable imposition of controls on heat-trapping gases like carbon dioxide.
In turn, 1 h of heat stress imposition revealed a significant up-regulation of most genes examined (with the exceptions of GR, NR, GCS and DREB), greatly ameliorated after progressive heat stress exposure.
For example, expression of StNAC030 was induced after 4 h of salt stress imposition and maintained upto 24 h, whereas its expression was induced after 24 h of heat and ABA treatment (Fig. 9), corroborating the RNA-seq data (Fig. 7).
Interestingly, the protective effect of NaHS root pre-treatment on heat stress tolerance in terms of transcriptional induction of defense-related genes was recorded 4 h after stress imposition, reaching maximal induction levels for most genes examined (except for CAT, NR, GS and DREB) after 8 h of heat stress.
The lower H2O2 and NO contents during the early stages of heat exposure in NaHS-pretreated plants can be attributed to late accumulation of HSPs (after 4 h of stress imposition), since both active molecules function in parallel with HSPs biosynthesis.
But the imposition of guilt continues".
No imposition of your own perfectionism.
"The imposition of celibacy is the same.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com