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Although virus-induced gene silencing (VIGS) has been used to characterize genes involved in abiotic stress tolerance, currently available gene silencing and stress imposition methodology at the whole plant level is not suitable for high-throughput functional analyses of genes.
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Our methodology employs VIGS-based gene silencing in leaf disks combined with simple stress imposition and effect quantification methodologies for easy and faster characterization of genes involved in abiotic and multi-stress tolerance.
Nevertheless, the lack of an approved common safety methodology which would allow the industry to quantify the risk to be reduced still leads to the imposition of the highest safety integrity levels for technical solutions in most European countries.
"It's an imposition.
"It is an imposition on the taxpayers".
I think this is a tremendous imposition.
But their methodology varies.
Her methodology is simple.
"We have strategy, methodology.
Potato Methodology Q.
The methodology has varied.
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