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To ensure robustness of identification of proteins as characteristic of one or the other or both study groups we imposed stringent criteria (see Methods).
Because we used a limited number of germ-free piglets per time point and measured responses in a mixed population of cells, we imposed stringent criteria for selection of genes (>3.6-fold up- or down-regulation and a false-discovery ratio of less than 5%).
Its application is favourable in situations of imposing stringent selection criteria.
On the other hand, imposing stringent inclusion criteria in terms of study methodological quality would have reduced the potential availability of studies considered for analysis.
Alternative stratification-based methods that use all samples without imposing stringent selection criteria include the conditional haplotype method (CHM), the conditional genotype method (CGM) and the conditional extended transmission disequilibrium test (CETDT) (Cucca et al. 2001; Koeleman et al. 2000a, b).
However, the short optical wavelength also imposes stringent requirements on fabrication.
The Rule imposes stringent requirements for certification that in practice exclude most claims.
This constraint imposes stringent requirements on the stability of the graveyard orbit.
The settlement imposes stringent practical requirements on UC.
Due to the heterogeneity of our source dataset, we imposed very stringent criteria to discover genes characteristic of hematopoietic progenitors.
We identify subsets of these genes when we impose more stringent criteria for: a) ≤10,000 basepair distances between reproducibly-positive SNPs, and b) at least 4 nominally-positive SNPs per cluster (Table 1).
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