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In addition, these methods impose additional limitations by requiring either relatively inefficient blunt-end ligation during library construction or redesign of transposons for tailored expression of circular permutants.
The component size, weight, geometry and material impose additional limitations to the machine capability.
Kinetic and regulation constraints, not included in these models, impose additional limitations on metabolic capabilities (see also Additional file 1, section 1).
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Variability within the signaling cascade imposes additional limitations on the fidelity of the signal relayed by calcium [75].
Second, the historical nature of our cohort imposes additional limitations.
Moreover, PHWR is a coupled system which imposes additional limitation in tuning the controller since the output of one PHWR's zone affects the outputs of other zones.
Some places impose additional restrictions.
The Committee on Discipline may impose additional penalties.
So we need to impose additional constraints.
Some agents impose additional fees.
Moreover, surgical simulations impose additional modeling challenges.
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