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More important, spindle remnants are normally no longer present within the connective cytoplasmic strand and therefore there is no risk of accidentally removing chromatids from the oocyte.
Secondly, we demonstrate how the PSM can be used to derive probabilities for traditional sleep stages, as well as for the important spindle process, and thus to create a continuous sleep profile in physiologically meaningful terms.
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It is clear that light microscopy cannot fully answer such important spindle-related questions and it is certainly beyond the scope of this manuscript to do additional EM experiments.
STIL appears to be important for spindle positioning and mitotic progression [ 59, 138, 139].
However, when hBora is deregulated, it perturbs Aurora A functions that are important for spindle assembly.
To identify novel kinases important for spindle assembly, we used a high-throughput RNAi screening approach.
Plk1 co-ordinates a variety of cell division processes including centrosome maturation, recruitment of important mitotic spindle components and cytokinesis.
In the same way, this stretching could separate Aurora B from relevant checkpoint targets, such as BubR1/Mad3 [6], whose modification is important for spindle checkpoint arrest.
We also evaluated the effect of MGRN1 inactivation on the motor protein, dyenin1, as it is important for spindle pole assembly and orientation.
Mud is the Drosophila counterpart of nuclear mitotic apparatus protein (NuMA), which is known to be important for spindle formation and maintenance in vertebrate cells.
We previously reported that NuMA interacted with RNA export factor 1 (Rae1) during mitosis and this interaction is important for spindle bi-polarity formation.
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