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2) Some of the data do not support the interpretation of observed results, particularly with Nek1, and some simple but important control experiments are missing.
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At several instances however the clarity of presentation would still profit from further improvement and one essential control experiment is missing: I) Missing control experiment: Figure 4: The new data clearly strengthen the manuscript.
However, detailed damage patterns observed in experiments were missing.
2) If such a model can be obtained, an important control for the DLS experiment is missing: measuring the DLS signal for a tuncated CPX without the accessory helix in complex with SNARE_delta60.
Most importantly, this experiment is missing an additional critical control, song with natural patterns of inter-pulse intervals.
The authors report control experiments for general viscosity effects but such a protein specific experiment is missing.
But sometimes that single "proof-of-principle" experiment is missing.
Several important controls are missing: it would be important to check by WB that the pMEFs generated are actually KO for caveolin-1.
Second, important controls are missing.
Needless to say, thorough control experiments are vitally important.
This important control is missing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com