The diagnostic importance of immunohistochemical staining for major histocompatibility complex I and II antigens, for the p62 protein, and of the recently identified anti-cN1A autoantibody in the serum, are discussed.
One case of our study highlighted the importance of immunohistochemical staining prior to DNA extraction for mutational analysis.
This apparent importance of p53 staining as a potentially useful marker in salivary neoplasms empowers other findings of association between higher salivary p53 expression in salivary gland tumours and poor survival [ 17].
The role of EUS in the evaluation of submucosal lesions (e.g. gastrointestinal stromal tumours (GISTs)) is beyond the scope of this article but the development of EUS-guided core biopsy needles and the importance of immunohistochemical staining for c-kit mutations has emphasised the importance of EUS in the management of these lesions.
The importance of optimizing staining protocols, data acquisition and data analysis for better assay performance has been highlighted by a series of proficiency panels for MHC multimer staining that were designed and organized by the Immunoguiding Program of the Association for Cancer Immunotherapy CIPP) and the Cancer Immunotherapy Consortium CICC) (12– 12).
The importance of predominant cytoplasmic staining in oesophageal cancer remains to be determined, but may reflect an increased binding of cytoplasmic Hsp90 client proteins.
The two stains give somewhat different patterns of tissue differentiation in x-ray images, emphasizing the importance of testing different stains on each new kind of sample.
At Procter, Ms. Watson acknowledged, "we here analyze, and overanalyze, the importance of whiteness, brightness, stain removal" when it comes to consumers choosing among detergents, but they "don't think about it in those terms".
With the initial interpretations of these Gram stains as gram-variable, which resulted in misidentification of the organism by three different laboratories, NYSDOH initiated an effort to educate clinical laboratories in New York State about the potential difficulties in characterizing the organism and the importance of primary Gram stain interpretation.
There was at first some skepticism concerning the importance and significance of ANA staining patterns, but this was in large part due to lack of appreciation of the structure, function and location of intracellular micro-organelles.
