Therefore, if careful examination of the evolutionary record were to reveal a significant tendency for gene fusion events that conserve rather than modify existing protein-complex assembly pathways, this would strongly support the importance of ordered assembly for the formation of functional protein complexes.
Overall, these results demonstrate the role of protein complex assembly in evolution and provide fundamental insight into the biophysics and biological importance of ordered assembly pathways.
Together, these results reveal the intimate relationships among protein assembly, quaternary structure, and evolution and demonstrate on a genome-wide scale the biological importance of ordered assembly pathways.
The results confirm the importance of using assembly fixtures that closely resemble regular production processing.
The importance of the assembly and function of the V-ATPase complex during fermentation, was highlighted by the number of genes identified (Additional files 4 and Table 3).
The similarity of our model to the SatP fold is not only supported by mutagenesis data but also suggests the functional importance of multimeric assembly not revealed by co-evolution or conservation analysis alone.
This illustrates the importance of considering assembly size when comparing between datasets, such as the high mapping rates to the P. taeda v1.01 genome obtained for P. contorta, P. pinaster and P. radiata (Table 4).
Pre-processing of reads is of great importance for assembly quality, as previously demonstrated by the GAGE study [ 45].
Inclusion of the coverage-based quantification dramatically increased the predicted TE content in many species, underscoring the importance of using assembly-free methods to gauge TE content.
Because of the importance of macromolecular assemblies in the machinery of living cells and progress in the EM and image processing methods, EM has become a major tool for structural biology over the molecular to cellular size range.
