In general, the dynamics underlying the receptor cycle of Pex5p seem to be of importance because the import and export rates of Pex5p have to be balanced [ 55].
As a consequence, the control of import and export rates is one mechanism by which the glycerol content inside of the cell can be altered.
These results indicate that TFEB subcellular localization is much more dynamic than previously appreciated and that the net localization and optical detection of TFEB in either the nucleus or the cytoplasm is the result of the relative nuclear import and export rates, which are influenced by nutrient availability and mTOR activity.
"Further exacerbated by port closures, national import and export rates fall by approximately 5% within a fortnight of the disaster," writes the Centre for Risk Studies.
Sequence variation generates different binding affinities, which correlate to protein import and export rates and therefore nuclear concentration.
These data derived from HeLa cells indicate that the nuclear import and export rates of RPL27-GFP are different.
Here, we present a model for extracting the import and export rates from FRAP experiments of STAT5B-GFP in the steady state of unstimulated NIH3T3-EpoR cells.
Therefore, we focused on the nuclear import and export rates of unphosphorylated STAT5 with the goal to generate rates for the steady state in unstimulated cells that can be set to a fixed value in a larger pathway model.
Our results provide a procedure to link directly measured import and export rates of unphosphorylated STAT5B with data indirectly describing the nucleocytoplasmic cycling of activated STAT5B generated by biochemical experiments.
Biochemical data describing the phosphorylation dynamics of the pathway components after stimulation in combination with mathematical modeling can then serve to indirectly determine nuclear import and export rates for phosphorylated STAT5.
To determine the import and export rates α imp and α exp 39 FRAP data sets generated from cells expressing varying concentrations of STAT5B-GFP were fitted with eq. (23) described in the Methods section.
