Initially, using the microarray dataset and long-term follow-up clinical data, we assessed the implications of gene expression on HCC recurrence.
It has recently become apparent that the location of a CpG has important implications in the effect of DNA methylation on gene expression (24).
Although attention has been focused on diversity in gene expression and its association with genetic diversity, there are no studies performed in a single breeding program examining the implications that consecutive cycles of breeding have on gene expression variation and identifying the variability still available for future improvement.
Hypomethylated CpG sites which exert control on gene expression may have implications for methods such as methylation-specific PCR and array technologies which rely on the methylation status of a small number of CpG sites in order to determine a given genes methylation status.
Importantly, several QGRS were detected in the vicinity of the intron-exon junctions of the SLC6A3: Because G-rich quadruplexes are actively involved in alternative and tissue-specific regulated splicing events [75], their positional effect on gene expression and the implication for DAT function could be most profound.
Besides transcriptional regulation, it is becoming increasingly recognized that control of the post-transcriptional steps has substantial impact on gene expression with widespread physiological implications [1], [2].
Such findings have implications for understanding parental effects and genome-dosage effects on gene expression and phenotypes in polyploid plants.
The effect of this genomic arrangement on gene expression regulation, and on the functional implications for the gene pairs listed in Table 1 warrant further investigation through gene expression analysis and functional characterizations.
In addition to the insight this study provides to the effect of broccoli consumption on gene expression, we consider that our study may have broader implications.
A handful of others exist, but most are based on brain scans rather than on gene expression maps.
