In two instances, CD81 intron 5 and TSPAN14 intron 4c (Figures S5 and S6), we were able to compare the intron sequence in two closely related species which ruled out any implication of the intron sequence in the exon change of length.
To estimate the biological implications of the sequencing data in silico, we applied four different software tools that allow for the prediction of the deleteriousness on protein function of each somatic mutation and found ∼75% of the mutations classified as harmful by all four algorithms.
Although the sensitivity, limitations and potential confounds of DTI are well recognized, the additional observation of a lack of change for most patients in their clinical upper motor neuron score over time (not previously reported to our knowledge) may have implications for the sequence of pathological change in ALS, and understanding the variable regional spread of symptoms.
Paleosol stratigraphy, a technique commonly applied in basin margin settings to depict cyclic alluvial architecture on time scales of 10 100 ky, can be consistent with regional accommodation trends at even higher temporal resolution (1 10 ky), having strong implications for the sequence stratigraphy of late Quaternary, non-marine deposits.
We also provide evidence that each FFRP has significantly functionally diverged at the protein sequence level, and in subsequent sections we will explore the implications of this sequence level divergence on the function of each FFRP.> -wrap-foot> Upper triangle contains pairwise coefficient of functional divergence (θij) between FFRP i and j and the standard error (SE).
However, the suggestion that a low baseline ALC may be associated with poorer OS does have implications for the sequencing of therapy in future trials that combine ipilimumab with chemotherapy.
Cladistic markers used to exam functional implication of the observed high sequence divergence appear to constitute amino acids essential for secondary structure and function of β-barrel channel.
While the implication of low sequence identity between the bacterial and Populus UGPase isoforms on enzyme activity is yet to be clarified, the functional activity of AxUGPase has shown to complement cellulose negative mutant phenotype by channeling sugar substrate for cellulose synthesis [ 34].> -wrap-foot> Data represent means ± SE (n ≥ 3).
The ratio of the two (RatioNN) may suggest functional implications of the protein sequences surrounding the query position.
When discussing the structural implications of the observed sequences changes, more care should be taken to distinguish hard evidence from previously solved structures (give PDB IDs and references in figure or caption!), modeling, and hypothesis.
