Along with phenotypic anchoring to existing end points, it is essential that the use of toxicogenomics data in a screening or regulatory setting not depend exclusively on achieving complete understanding of the functional implications of individual gene, protein, or metabolite changes.
These reports (for example see Additional file 1: Figure S1G) allow users an intuitive view of the prevalence of HGT-derived genes and the evolutionary, ecological and functional implications of HGTs at levels of individual genes, gene families, whole genomes and multiple phylogenetically related genomes.
Thus, the transcriptional profile of CLL can be reduced from a daunting number of individual genes to a handful of meaningful pathway annotations with important biological and clinical implications.
Already, some useful data has emerged from this effort, as the mappers have published various gene sequences -- the coded text of individual genes.
The evidence that this struggle is being waged at the level of individual genes is accumulating, if mostly circumstantial.
An implication of these observations is that it may not be wise to rely on single key genes as modulators of the observed effects, as the statistical reliability of any individual genes is moderate, compared to the robust statistical significance of coregulated or functionally-related gene groups.
One federal laboratory has sequenced the DNA of several individual genes in various Ames stocks without finding any differences.
Here, we present a high-throughput screening (HTS) strategy to systematically evaluate the implication of each individual E. coli gene in bacterial conjugation.
But geneticists must analyze thousands of genomes to isolate the effect of any individual gene.
