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In the second part, we corroborated the results (obtained by the HRM method) using the standard Sanger sequencing technique.
A mutational analysis, by Sanger sequencing technique, was performed in the Bologna reference laboratory in all cases of loss of CCyR or MR3.0.
Interestingly, two samples were detected as WT in the HRM technique while the Sanger sequencing and the THxID™-BRAF test found a V600E mutation.
Then in 1977 Dr. Sanger greatly improved his strategy and developed the "Sanger Sequencing" approach, which earned him a Nobel Prize (Sanger et al., 1977).
The pyrosequencing technique was found to be superior to the microscopy method and more rapid than the Sanger sequencing method.
The Sanger sequencing results are shown in Figure 2a d.
Obviously the Sanger sequencing was developed based on Wu's primer-extension method.
PCR amplification and the Sanger sequencing were carried out as described by Guo et al. (2015).
The Sanger DNA sequencing technique has been and still is the method of choice for de novo sequencing of complete genomes [ 1, 2].
The composition of bacterial communities is typically studied by implementing clonal Sanger sequencing of 16S rRNA PCR products [ 1- 4].
The Sanger sequences of chicken, turkey and zebra finch DNA have long been available.
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