In this report we implemented a simple procedure based on a modification of the Rapid Amplification of 5' complementary DNA ends (5' RACE [20], [21]) named Directed Amplification of TSSs (DMTSS), and a high throughput pyrosequencing strategy (HTPS) with Roche's 454 GS20 instrument, to experimentally determine as many TSSs as possible in the E. coli K-12 genome.
Grape also implements a simple procedure to identify chimeric RNAs independently of those cases found by Cufflinks, which can be used if the input is paired end reads.
Implementing a simple screening procedure appears suitable to increase the routine recording of mental health problems in a stop smoking service, which is an essential step to ensure services can be tailored and delivered appropriately to the client group.
Respondent selection within a household involved implementing a simple randomisation procedure, the so-called next birthday rule.
Since the PLAIN mechanism consists of a single message from the client to the server, it is implemented as a simple procedure rather than a SASL protocol context.
The proposed approach can be implemented as a simple procedure to design structures with repetitive units, with the advantage of simplifying numerical simulations and reducing the computational cost.
This is done by implementing a simple queue.
Furthermore, we implement a simple processing procedure based on Short-time Fourier Transform (STFT), by which strong time-frequency domain response of the tags is clearly seen at those designed resonant frequencies, and disappear when tags are not inserted.
Thus, the proposed method may need further improvement, but in the short term, it makes possible to implement a simple and general procedure that accounts for these new selection practices in BLUP evaluations at the national level.
It should be noted to highlight the value of testing the AEC system, which is a simple procedure to implement that provides valuable information on the overall performance of the mammography system.
