This information is collected by each communication node from the latest received beacon sent by its preceding vehicle, essentially implementing a sample-and-hold mechanism.
This problem has been addressed by Erlich et al. [14] by implementing a sample-pooling pattern combined with tagging of the pools and Galan et al. [16] by combination of tags in the forward and reverse PCR primers.
We then show the possibility of combining existing frameworks of DNA computing to implement a sample hybrid controller for a molecular robot.
Because of this, the study has implemented a sample size re-estimation, which will occur 1 year later after the 400th patient has been enrolled or once 2000 patients have been enrolled.
Rödig and Siebert implemented a sample-changing wheel to provide fresh sample after each reaction [ 16].
However, this conclusion has been challenged [ 13, 44], because in practice, it is difficult to design and implement a sampling protocol free of methodological bias.
Our work, however, differs from that presented in reference [ 9] in that our dataset is (i) unfettered by ascertainment bias or assumptions of a two-species model (see electronic supplementary material), (ii) uses 80K more loci and (iii) implements a sampling and analytical design intended to specifically test hypotheses of hybrid origins for the different Canis types.
Among other important issues, implementing a Gibbs sampler involves choosing a sampling strategy, deciding the number of chains to be generated, and defining the initialization values, length of the burn-in period, and number of cycles needed to ensure a representative sample from the marginal distribution of interest [ 40].
Implementing a cluster sample of local registration offices might be an option.
The chapter concludes with a discussion of the difficulties encountered and the considerations that have to be made for implementing a new sample preparation technique.
