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Currently implemented workflows include those for adapter trimming, read mapping, peak calling, motif discovery, transcript assembly, variant calling and chromatin conformation analysis.
The implemented workflows currently use BLAST+ (Camacho et al., 2009) version 2.2.28+ using any of the blastn, megablast or tblastx algorithms and nucleotide LAST (Frith et al., 2010) version 320.
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Figure 1 shows the implemented workflow.
The implemented workflow is microarray platform independent and is presented as a web-based service.
Instead, it is a telemedicine network, with a distributed archive, with an implemented workflow and quality improvement cycle.
All data, including the exact input data used, intermediate data, third party data, output data, and their provenance, were captured and preserved within our implemented workflow.
The experimental process using Isotopo consists of three major steps: data preparation, analysis and visualization as shown in Figure 1, following the implemented workflow as shown in Figure 2a.
Based on these guidelines, users should be able to implement workflows using irreversible image compression.
Implementing workflows using UNIX scripts and pipes of command line programs is very lightweight while still powerful.
We are currently working on expanding our use case basis by implementing workflows in the fields of metagenomics, comparative genomics, and haplotype analysis according to our methodology.
We expect that profiling may enhance the quality of species occurrence data but may also be useful when implementing workflows for geo-correction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com