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We note that automated sample handling and image analysis can be easily implemented using standard methods, as we and others have shown previously, and that AFM technology has progressed to the point that image capture rates can approach that of optical microscopy.
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The mathematical system of equations is numerically implemented using standard methods for spatial discretisation and time advancement.
Alkaline phosphatase wholemount in situ hybridisation experiments were performed using standard methods as described previously71.
Chemical analyses (Supplementary Table S2) were done using standard methods, as previously described9.
All NTS specimens were tested by RT PCR for influenza A and B viruses using standard methods as described elsewhere7,8.
Previous protein localization prediction methods have been implemented using standard machine learning algorithms with parallel architecture as a common practice in computer science [24 26].
All methods were implemented using standard SVM leave one out cross-validation in balanced data sets.
Our method could be readily implemented using standard software (with code supplied) to produce an overall better model fit as measured by BIC and deviance.
This stepwise method can be implemented using standard statistical software.
Our method is based on inverse kinematics and it can be implemented using standard robotics libraries.
All methods were fairly easy to implement using standard statistical software.
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