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A function is implemented to read the patterns and store them in a vector.
Most pipelines save data in a traditional file system since most analysis tools are implemented to read and write files in POSIX file systems.
A classifier is implemented to read the seismic registers at 30-second intervals, extract the most relevant features of each interval, and classify them into one of the three kinds of signals considered as most representative of this particular volcano.
For example, a columnar storage system may improve I/O performance, but many analysis tools are implemented to read and write regular files and hence cannot directly benefit from columnar storage.
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Tag Find uses a fastq file as input, and was implemented to discard reads shorter than a user-defined value, after tags elimination.
We evaluated the effect of mapping quality (mapQ) on the accuracy of predictions and found that poorly mapped reads (mapQ < 15) are only found in FP (Additional file 1: Figure S1), so a quality filter was implemented to exclude these reads from subsequent analyses.
Finally, 'galign' is currently implemented to detect sequence reads containing only zero or one mismatch.
Tophat [ 36] (v2.0.4) was implemented to align the reads, by default, and Cufflinks (v 2.0.2) was used to assemble transcripts with the –g parameter.
The assembly of long reads generated using the Oxford Nanopore MinION® instrument is challenging as existing assemblers were not implemented to deal with long reads exhibiting close to 30% of errors.
As existing assembly softwares were not implemented to deal with long reads with a high error rate, we developed a method based on a combination of two sequencing technologies: Oxford Nanopore and Illumina, to produce long and accurate synthetic reads before assembly.
Recently a new algorithm, Burrows-Wheeler Aligner's Smith-Waterman Alignment (BWA-SW), has been designed and implemented to align with gaps long reads such as 454 reads (~200 bp or longer) to the reference genome with higher accuracy and a faster speed than BLAT [ 28].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com