In this study we implemented the sFIDA assay on an automated platform to enhance robustness and performance of the assay.
By contrast, when we implemented the cBED assay formulae using the externally measured long-term FPR of 0.0560 [6], all four cBED assay-based HIV incidence estimates were significantly lower than the longitudinal estimates (Table 2).
Furthermore, when we implemented the cBED assay formulae using the lower bound or upper bound of the 95% CI of the locally measured long-term FPR (0.0100 0.0266), the cBED assay-based HIV incidence estimates did not differ significantly from the estimates based on longitudinal measurement.
In this study, we implemented the sandwich assay.
We then implemented the pseudovirus assay to measure neutralizing antibodies in clinical samples with known HI titers for subtype H7N9.
Furthermore, we implemented the chemotaxis assay to confirm that priming BMSPCs with LL-37 from patients with MI increases their mobilization to low, yet physiological, levels of SDF-1 (2 ng/mL) [ 65].
In order to test the effect of the mutations on protein folding, we implemented the GFP-folding assay.
However, if capping were implemented before the assay, it would then be impossible to recover a failed coupling.
When the amount of available genomic DNA was below 250 ng, a first whole genome amplification (Qiagen, REPLI-g Mini Kit PN: 150023) step was implemented to the assay.
Two signal enhancement techniques based on isothermal circular strand-displacement polymerization reaction, cyclical nucleic acid strand-displacement polymerization (CNDP) and cyclical common (nonnucleic acid) target-displacement polymerization (CCDP), were employed to implement the amplification assay for p53 gene and PDGF-BB, respectively.
