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DEJ carried out the experiment design, implemented sample preparation and conducted the microbiological sampling.
By using the implemented sample preparation routine, each plate was prepared in less than 15 min. This procedure was done using tailor-made routines under the control of Hamilton method manager software, version 5 (Hamilton) (see the Supporting Information).
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Six implemented sampling criteria are detailed in a tutorial and illustrated with graphical examples.
Nested sampling (NS) is a highly efficient and easily implemented sampling algorithm that has been successfully incorporated into Bayesian inference for model updating and model selection.
It is still unclear as to whether the hermaphroditism observed in the laboratory was induced by our manipulations or whether it is a common characteristic of this species which, due to its rarity, was overlooked by the implemented sampling program.
The authors implemented sampling plan 2 into their model.
We have applied the implemented sampler to the inference of evolutionary and demographic parameters from simulated sampling data to ensure that the inference scheme is capable of recovering the truth in situations where this is known a priori.
Here we used a newer version of Migrate with high signal data and found no evidence of lack of convergence as repeated runs were highly consistent using the implemented sampling strategy.
It implements sample shuffling.
The number of samples or replicates in a typical RNA-Seq experiment is usually small, thereby excluding the application of nonparametric methods that implement sample permutations.
There is now extensive experience in implementing such sentinel mortality surveillance systems - Health and Demographic Surveillance Sites (HDSS) with VA - and growing experience in implementing sample registration with VA (SAVVY) (17, 22 ).
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