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The implemented quantification algorithm and Hadoop platform are also introduced here.
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Later on, the quality control step was implemented with quantification by PicoGreen method on 96-well plates and high throughput microfluidic capillary electrophoresis system for library profile analysis (LabChip GX, Perkin Elmer, Waltham, MA).
Protein concentrations were calculated with the implemented "Protein Quantification with BCA" protocol in SoftMax Pro software (Version 5.3, Molecular Devices).
The raw data were obtained using Rotor-Gene 6 software (Corbett Life Science, Sydney, Australia) via the implemented comparative quantification algorithm.
This improvement can be easily implemented in quantification methods [ 2, 7] and it can bring methodological and technical improvements for intra-operative 5-ALA-induced fluorescence microscopy.
Recently, researchers implemented comparative quantification of global miRNA transcriptome in endothelium derived from atheroresistant and atherosusceptible arterial regions in order to identify miRNAs whose expression could distinguish between resistant and susceptible regions.
However, a major challenge of implementing LRE quantification is the labor intensive nature of the analysis.
Foremost is the difficulty of implementing absolute quantification, due to the necessity of constructing target-specific standard curves [4].
Paramount is the difficulty of implementing absolute quantification due to the necessity of constructing target-specific standard curves [1].
An extensive help set also provides an in-depth introduction to LRE, in addition to guidelines on how to implement LRE quantification.
In addition to imparting exceptional quality control capabilities, the ability to exploit bacteriophage lambda genomic DNA (lambda gDNA) as a universal quantitative standard provides a simple and reliable approach to implementing absolute quantification.
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