One benefit of the systems is that they can be made of transparent materials such as rubber silica, Plexiglas or glass and as a consequence they can easily be implemented onto microscopes to be combined with suitable read-out techniques.
The HiLo technique was easily implemented on the microscope Olympus BX51 by adding a dual port tube (Olympus, U-DP) right before the microscope illumination path, thus allowing the user to choose between speckled laser light (for HiLo microscopy) and a monochromator light (for calcium imaging, see below).
Confocal microscope implemented with our flow chamber proved to be a promising tool for time-lapse investigation of cell biomaterial interactions.
In order to display the real performance of the algorithm if implemented in the microscope, we compare the results of the matrix algorithm and the presented algorithm on real data of a calibration grating.
Results of the analysis tools implemented in the MicroScope pipeline are stored in specific relational tables, together with the primary data used by the methods.
We designed a new CGWS scheme based on a Linnik interferometer and a SLED light source, which is able to compensate dispersion automatically and can be implemented on any microscope.
Starting with the description of the flow chart of the annotation processes implemented in the MicroScope pipeline, and the development of traditional and novel microbial annotation and comparative analysis tools, this article emphasizes the essential role of expert annotation as a complement of automatic annotation.
In summary, Raman spectroscopy has been implemented through a confocal microscope and used to successfully classify oral bacteria in hydrated biofilms of one or two species.
In the work described here, Raman spectroscopy implemented through a confocal microscope has been used to distinguish between two species of streptococci, Streptococcus sanguinis and S. mutans, grown in biofilm form.
We compared genomes of A. mediterranei (CP002896.1), C. diphtheriae (BX248353), C. glutamicum (BA000036), M. tuberculosis (AL123456), M. smegmatis (CP000480), N. farcinica (AP006618), N. cyriacigeorgica (FO082843), R. equi (ADNW00000000) and R. jostii (CP000431) using various graphic tools implemented on the MaGe Microscope platform and ACT.
