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Statistical comparisons to evaluate differences in the genetic diversity indices were calculated using permutation procedures as implemented in using F STAT version 2.9.3.2 [ 49].

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All simulation software was implemented in-house using IDL ITT Visual Information Solutionss, Boulder, CO, USA).

The dispersion analysis in relax is implemented in Python using NumPy and the GUI using wxPython.

The test was implemented in RAxML using the same data partitions used to estimate our phylogeny.

Sequences were classified using the RDP classifier program implemented in mothur using the bayesian method.

All algorithms used for our comparison were implemented in C++ using OpenCV library, the compiler is gcc version 4.4.3.

The design has been simulated using the Verilog programming language and implemented in hardware using FPGA.

The algorithm was implemented in R using mpiR package to enable parallel processing using HPC Wales computer cluster.

Primer pairs for the identified microsatellite loci were designed using the Primer3 software [ 48] implemented in Msatfinder using default parameters.

Implemented in python using the GNOME UI package and GTK2.

Random forest was implemented in R using the randomForest package V.4.6 1258.

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