The procedure used to create the respiratory-deficient strain S. cerevisiae 311 introduced unknown genetic alterations that could not be directly implemented in the wild-type metabolic network.
In any case, the biosafety system implemented in the company was working mainly to prevent infections from wild animals but not from humans.
Firstly, in the wild sample we used a sibship reconstruction approach implemented in the software program COLONY (Wang, 2004) using nine microsatellite loci to identify pedigree structure.
The difference between wild and cultivated longan germplasm was measured using Fst, as implemented in the same program.
Free energy changes between the wild type let-7a targets and edited let-7a targets were calculated by RNAeval algorithm implemented in the Vienna RNA package.
ΔK values of all rice wild relatives for different values of K tested, following Evanno et al. (2005), implemented in the STRUCTURE HARVESTER software (Dent and VonHoldt 2011).
We conducted an in-the-wild week-long study that implements Maker activities designed with 3rd, 4th, and 5th grade teachers during science classes.
Simulation of the metabolic network model along with data preprocessing were implemented in GAMS for the wild-type (control condition) and lpdA mutant (perturbed condition), separately.
Current assessment of biomaterial biocompatibility is typically implemented in wild type rodent models.
The structure was determined by the automated molecular replacement method Phaser as implemented in CCP4 (20), starting with the wild-type (wt) RB69 pol structure of the ternary complex [PDB entry 1IG9 6)].
Compulsory surveillance programmes for avian influenza (AI) have been implemented in domestic poultry and wild birds in all the European Member States since 2005.
