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Significant rate differences were identified in the case of three of the partitions (16S rRNA, control region and S7 intron), which prompted us to estimate the ages of divergence events among different seahorse lineages by means of the Bayesian relaxed clock method for multiple genes implemented in the programmes ESTBRANCHES and MULTIDIVTIME [ 58].
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Allelic richness was estimated using the rarefaction algorithm implemented in the programme ADZE [39].
Other evaluations of similar interventions implemented in the programme setting have reported wide variations in intervention coverage.
The methodology applied here is described in Perez-Enciso et al. [ 23] and implemented in the programme QxPak.
To increase the enrolment rate for girls in primary and especially in secondary school, a strong school component is implemented in the programme.
The significance of the differences between the controls (28°C) and the heat-shock treatments (34°C) was determined with a strong randomization (iteration) test as implemented in the programme.
This was implemented in the programme METAL (http://www.sph.umich.edu/csg/abecasis/Metal/index.html; Willer et al. 2010).
RPB1 DNA sequences were aligned on the basis of an RPB1 amino acid sequence template, implemented in the programme DAMBE [ 73].
The Beaumont method [ 42] implemented in the programme MSVAR 0.4 assumes that a stable population of size N1 started to decrease or increase ta generations ago toward the current population size N0.
The AFLP data were analysed using Principal Coordinates Ordination (PCO) as implemented in the programme PAST (Øyvind Hammer and David Harper, available from http://folk.uio.no/ohammer/past/) using the DICE similarity metric.
N e V was estimated using the pseudo-maximum likelihood method implemented in the programme, MLNE v. 1547 (Wang, 2001) and the moments-based temporal methods of Nei/Tajima (Nei and Tajima, 1981), Pollak (Pollak, 1983) and Jorde/Ryman (Jorde and Ryman, 2007) implemented in NeEstimator v. 2.0 (Do et al., 2013).
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