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Values of FST were also estimated for each pair of populations using Weir and Cockram's Θ estimator (Weir and Cockerham 1984) implemented in GENETICS software (Belkhir et al. 2000).
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Allele and genotype frequencies were estimated by direct counting and deviations from Hardy-Weinberg equilibrium (HWE) were tested, in cases and controls separately, using the exact test as implemented in R Genetics package (version 1.2.1) [73].
Linkage disequilibrium was measured using D' and r 2 statistics as implemented in the genetics library.
We next tested each SNP for deviation from Hardy-Weinberg equilibrium (HWE) [ Salanti et al., 2005] using Fisher's exact test as implemented in the genetics package [ Warnes et al., 2007] in R, and checked the validity of the missing-completely-at-random (MCAR) assumption by calculating the Fisher test P value for the 2 × 2 table of case/control responses vs. missing/nonmissing data.
Laboratory modules were implemented in upper-division genetics and cell biology courses using cassava as the central theme.
Mainstreaming should be implemented in collaboration with genetics, who should continue to see any individuals found to have mutations, and any complex cases.
Phylogenetic trees were constructed by using nucleotide alignments, the Jukes Cantor algorithm, and the neighbor-joining method implemented in molecular evolutionary genetics analysis (MEGA) software (12).
The series of laboratory exercises on transcriptional response to abiotic stress in maize was implemented in the introductory genetics course and in the upper-level biotechnology course and as an approach to introduce summer research students to RNA-seq analysis.
Dendrograms were constructed from the genetic distance matrices of both dataset 1 and dataset 2 using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) algorithm implemented in molecular evolutionary genetics analysis (MEGA), version 6 [ 21].
Expression estimates was calculated using gcRMA implemented in GeneSpring 7.1 (Silicon Genetics, Redwood City, CA, USA).
All calculations of the r statistic were implemented in the R package "genetics" (Warnes et al. 2011).
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