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To minimize the possibility that genetic modification of the Rtn4r locus could affect expression of the two neighboring genes, we designed and implemented a gene targeting strategy (Fig. 2B F) to replace exon II of Rtn4r with the self-excisable selection cassette that includes the neo gene selectable marker (pACN) [40].
We implemented a gene expression analysis of sesB flies using procedures identical to those used previously to analyze tko 25t.
To facilitate biological interpretation of the users' results, we have locally implemented a Gene Ontology (GO) (Ashburner et al., 2000) enrichment tool based on GO::TermFinder of Boyle et al. (2004).
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So in addition to the normal single marker tests, we have also implemented a gene-level test that groups all carriers of previously unreported and likely functional variants in a given gene.
As an optional step towards downstream analysis, ADMIRE additionally implements a gene set enrichment procedure.
We also explored the interdependency of our variables by implementing a gene interaction network model in GALGO.
In addition, we offer a set of Perl modules that can be utilized by other scientists as a generalized framework for implementing a gene page in MediaWiki, as well as a customizable genome browser for visualizing large-scale genomic data using JBrowse.
It was found that variation in the life history and mating characteristics of a population could alter the reproductive values of released males and females, and thereby affect the effort needed to successfully implement a gene-drive project.
One way of implementing a gene- and tissue-) specific component could be througene- andc changes in the local chromatin specifice that inducomponentn locouldariations in the polymerase elongation rate.
We implemented a weighted gene co-expression network analysis (WGCNA) [ 27] to identify modules of co-expressed genes during different stages of development in Drosophila melanogaster.
10 We have implemented a new gene set enrichment analysis method, FDR-FET, which was first described by Ji et al 11 in a transcriptional profiling study of compound dose responses.
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