In this study, we implemented a constraint-based sampling approach on an updated genome-scale network of yeast metabolism to systematically determine how EM level variations are linked to global changes in intracellular metabolic flux states.
To improve the detection specificity, we implemented an additional constraint related to the conserved head-to-tail connection module organisation observed among most of the phages.
We implemented a set of constraints that resulted in key improvements in the physiological response of S. cerevisiae under anaerobic fermentation.
While the underlying cellular objectives cannot be directly deciphered from these data, the changes in metabolite profiles can be implemented as a constraint to be satisfied, through formulation of a pseudo-reaction, grossly similar to the use of non-growth associated biomass objectives in bacteria [ 25].
In the present study, a new method to implement a strength constraint for the FBB method is proposed for the simultaneous optimization of more than two laminates (such as a panel and stiffeners).
Subsequently, an optimal fuzzy system (op-FS) is designed to fully implement a parameter constraint mechanism so as to guarantee the system stability converging to the desired state even if the UAD variability rate increases in a given range.
Here, we did not, but certainly can, implement a hard constraint to disallow any infeasible dihedral angles.
Because we implement a viability constraint that all genes must remain regulated, the effect of increasing the binding site length, at some point, is masked by the inability of pathways to lose any more binding sites.
For these initial measurements in humans, we implemented a protocol that imposed constraints on the subject pool and the acquisition parameters.
This idea was first introduced into connectionism by Hopfield and Tank [ 21] and implements a soft-constraint satisfaction.
As explained in the main text, VS-SAIM's design is based on the principle of minimization of an energy function in order to implement a soft-constraint satisfaction.
