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In our implementation, the sample rate of each PIR output is 25 Hz.
As the study aimed to evaluate intervention use and implementation, the sample consisted only of practitioners and staff within the trial.
In the gap-fill padlock or MIPs implementation, the sample DNA is fragmented by shearing and used in the bipartite circular structure to provide a template for the probe DNA to be extended by gap filling and converted to a closed circle.
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Availability and implementation: The sampler has been implemented as a publicly available BEAST 2 package that is distributed under version 3 of the GNU General Public License at http://compevol.github.io/MultiTypeTree.
The non-randomised trial (Gordon 2005) has an unclear risk of bias in terms of its design and implementation, but the sample size of this study is less than the optimal size so the evidence on the effects of handing out application materials in hospital EDs is of low quality (Summary of findings 2).
In the following two subsections, we describe the hierarchical Bayesian model, and, in the last two subsections, we describe the implementation of the sampling from the posterior distribution.
An additional issue is that no implementation of the sampler has been made widely available.
The code for simulating the implementation of the sampling plan was originally written in R (http://www.r-project.org) but for this paper the code was translated into plain C for improved efficiency.
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2334/14/87/prepub wouldulikeike to thank the colleagues from the Department of Epidemiology and Health Monitoring, Robert Koch-Institute, for providing valuable support with the design and implementation of the sampling frame.
Our implementation of the sampling algorithm (see http://compbio.cs.princeton.edu/fcsampling for source code available under a GNU public license) also includes a variant that uses a Metropolis criterion to accept or reject moves.
In our current implementation, we set the sample size to 10%, for performance and scalability reasons.
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