For choice of pooling method, we use max-pooling here.
The four types of pools (RSP, CSP, RP, and CP) are screened with Illumina GoldenGate genotyping techniques [ 9] in this new implementation of the pooling strategy.
However, for actual implementation of creative ideas, the most creative ideas must be recognized and selected from a pool of ideas.
A computational algorithm was designed for this implementation of the 5-D pooling strategy, which was integrated into a newly developed software tool, FPCBrowser, for analyzing pooling data.
Meanwhile, in the implementation of direct marketing, intermediaries who pool RES plants play an important role [28, 82].
A new implementation of 5-D BAC clone pooling strategy employing both the GoldenGate assay to screen BAC pools and the use of previously assembled BAC contigs is suggested.
The new implementation of the 5-D clone pooling strategy employs the GoldenGate genotyping assay to screen BAC clones in 4 types of pools, RSP, CSP, RP and CP (four dimensions), which replace PCR screening and verification for positive plates in RSP and CSP and for positive clones in RP and CP in the previous implementation [ 9].
The implementation of the 5D BAC clone pooling strategy is a highly efficient, low cost, and rapid approach to screen a BAC library.
A new implementation of 5-D BAC clone pooling strategy employing both GoldenGate assay screening and assembled BAC contigs is shown here to be a high-throughput, low cost, rapid, and feasible approach to screening BAC libraries and anchoring BAC clones and contigs on genetic maps.
