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To verify the model, its predictions for a laser pulse with a duration of ∼150 ns and a wavelength of 308 nm were compared to those of a phase-field implementation of melting laser annealing by La Magna et al. The two models show a good agreement for the melt depth, melt duration, and melt front dynamics.
However, real-time PCR used in this study, by implementation of melting curve analysis, increased reliability of results and solved the problem of SYBR Green I, which binds to nonspecific double-stranded DNA [ 14].
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