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Implementation of immunostaining: AW, SB.
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Reproducibility of immunostaining techniques and microscopical evaluation are prerequisites for a standardized and reliable quantitation of immunostaining intensity.
Category A scored the intensity of immunostaining as 0 (no immunostaining), 1 (weak immunostaining), 2 (moderate immunostaining), and 3 (strong immunostaining).
SX performed statistical analysis of immunostaining experiments.
Background DAB signal was adapted for comparable range of each type of immunostaining prior to evaluations.
In each section, expression, intensity of immunostaining and percentage of labeled cells were analyzed.
Detection of immunostaining was performed using a Dako Rabbit Envision HRP polymer kit (Dako, Cat. #51699).
YC participated evaluation of immunostaining.
The intensity of immunostaining was evaluated as follows: 0 = without immunostaining; 1 = weak immunostaining; 2 = strong immunostaining.
The cellular location of immunostaining was divided into three groups: 0 = without immunostaining; 1 = cytoplasmic and nuclear immunostaining; 2 = membranous immunostaining.
Due to high suspicion of eAML, immunostaining panels included melanocytic markers, cytokeratins and RCC-specific markers.
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