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Results of the present study, together with recent findings on distinct trade-offs between direct (cyanogenesis) and indirect defences (VOCs) of lima bean, strongly suggest implementation of cyanogenic features in further studies on lima beans' overall defence in nature.
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Analysis of individual leaf cyanogenic features revealed a significant negative correlation of leaf damage and HCNp (according to Pearson's correlation: r = −0.567, P<0.001; n = 46; Fig. 2).
It does not require implementation of new features.
Thorough planning results in the successful implementation of SharePoint features.
We validated our implementation of these features in GiA Roots against a MATLAB implementation.
Use of JEnsembl has both validated the API and driven implementation of new features as required.
Alternatively, the results of this field study suggest that the observed quantitative variability of cyanogenic plant features is under genetic control as has been observed for many other cyanogenic plant species [e.g. 30], [36] [38].
These leaflets with defined cyanogenic features therefore allowed for utilization of naturally occurring HCNp variability for functional analyses of its efficiency in anti-herbivore defence under experimental feeding trial conditions.
Before natural variability of cyanogenesis may be used for functional analysis of its defensive efficiency in herbivore-plant interactions, the quantitative variation among cyanogenic features must be known.
This high degree of genetic control of cyanogenesis in wild type lima bean was confirmed in our cloning experiments where plant material with defined cyanogenic features was generated for feeding trials.
We used natural chrysomelid herbivores and clonal lima beans with known cyanogenic features produced from field-grown mother plants.
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