The benefits of implementing high throughput sequencing in the clinic are quickly becoming apparent.
For instance, Saunders et al. [ 12] and Duplessis et al. [ 13] both implemented high throughput computational methods to characterize the effector complements from the fully sequenced rust fungi PGT and Melampsora larici-populina.
Methods such as this are an important step in implementing high-throughput and inexpensive aggregation assessments that can be incorporated into a simple and actionable manufacturability risk.
The robotically implemented high-throughput trichloroacetic acid (TCA) precipitation assays, measuring the incorporation of (α-P) rUTP into TCA-insoluble products, were carried out exactly as previously described [ 19, 20].
CDC laboratory scientists implemented high-throughput laboratory capacity by using robotics and collaborated with private industry to promote development of lateral-flow assays to detect Ebola in point-of-care settings within 30 minutes after a finger stick or oral swab (6 ).
Second, the NTP needs to develop and implement high- and medium-throughput screens to identify and understand potential targets for environmentally mediated disease.
ArQule, Inc. was one of the first companies to develop and successfully implement automated high throughput parallel synthesis of several hundred thousand single compounds per year in a spatially addressable format.
In particular, a specifically designed microfluidic device has been implemented for high throughput genotyping and its stability has been validated on a set of 150 thermally cycled biochemical reactions.
Planar imaging is the simplest method, being easy to implement and offering high throughput.
This method does not require any PCR-based amplification, can monitor the abundances of multiple transcripts within a single RNA sample, and was successfully implemented into a high throughput screen of transposon mutant library members to detect isolates with altered mRNA turnover properties.
