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Along this line of research, new approaches are expanding to implement flow cytometry data, examples being fate mapping studies, functional assays, and genome-wide analyses (79– 82).
It can be implemented in the laboratory to provide a convenient adjunct to conventional functional assays for determining the overall differentiation state of an ES cell colony following LIF removal.
We have implemented a generalized and logical lincRNA candidate selection process that leverages a collection of cell-based functional assays, RNA-sequencing data and computational analyses.
Moreover, functional assays using Saccharomyces cerevisiae revealed that the Y424H substitution did not alter function of CHEK2 protein.
The excellent assay quality and correlation between structural and functional assays validate this method for large-scale HTS campaigns.
Finally, we applied a series of functional assays to validate the sequencing results.
V.R., D.K., M.F.R., T.A., and P.D. performed functional assays for candidate genes.
Confirmation is gained by metabolomics, mRNAseq, and functional assays of cellular glucose uptake after BCG vaccinations.
Day 3.5 EBs were dissociated for downstream functional assays or gene expression analysis.
Coulombel, L. Identification of hematopoietic stem/progenitor cells: strength and drawbacks of functional assays.
M.N., N.S., F.W. and S.P. performed and analyzed the functional assays.
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