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Clinical results are highly variable and can be difficult to compare between studies because of huge variations in cell source, preparation and implantation methodology.
In a preliminary pilot experiment (see below), the implantation methodology (including supportive measures during implantation) was established and practiced until the technique was optimized and highly reproducible.
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The advantages of an in ovo model compared to animal models included simple methodology of tumor cell implantation, economical reasons, and high survival rate of embryos.
In a preliminary pilot experiment, the anaesthesia and surgery methodologies, including supportive measures during implantation, were optimized and practiced in several wt and tg6 mice (data not shown).
Allograft pulmonary artery tissues decellularized with an anionic detergent/enzyme methodology were tested in a long-term implantation model that used arterial wall repairs in the great vessels of juvenile sheep.
The proposed antenna design methodology can be applied to optimize antenna for several implantation scenarios and ISM band applications.
Thus, using the resources of experimentation and techniques of teaching methodology (role-playing), we conducted a practical training of skills in implantation and management of the IABP for doctors, residents and nurses.
The 2i methodology results in ES cell lines that are transcriptionally and epigenetically similar to pre-implantation epiblast [ 20- 22].
This methodology includes (1) isolation, characterization and culture of ECFCs, (2) isolation, characterization and culture of MSCs, and (3) implantation of ECFCs and MSCs, in the presence of FGF-2, into immunodeficient mice to generate perfused vascular networks.
Saunders et al. utilized the in situ gelling methodology to introduce load bearing scaffolds into damaged intervertebral discs (IVDs) as an alternative to traditional, high-risk spinal implantation surgery for IVD repair.
implantation failures.
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