Following subcutaneous implantation in the back of immunocompromised mice, bioprinted constructs generated organized artificial muscle tissue in vivo.
Tissue engineered cell-seeded constructs with poly(3)hydroxybutyrate (PHB) induced ectopic bone formation after implantation into the back muscle of rats.
In this study, we propose to evaluate the remodeling, vascularization, and functionality of a chitosan-based, tubular neuromuscular tissue on subcutaneous implantation in the back of athymic rats.
Four piezoresistors are formed by ion implantation on the back side of a thin Si membrane, while on the front side a 380 μm high Si mesa is produced by subtractive dry etching (deep reactive ion etching).
Before implantation in the back of SCID mice carriers were freshly loaded with 2×105 expanded human MSC or loaded with cells and kept under osteogenic conditions for two weeks in vitro.
After anesthesia, the mice were subjected to subcutaneous pump implantation in the back with an osmotic minipump (Alzet) filled with phenylephrine (PE, 30 mg·kg–1·dissolvedolved in 0.5 mmol/L ascorbic acid) or vehicle (0.5 mmol/L ascorbic acid), the incision was closed with 2 wound clips.
After subcutaneous implantation into the backs of mice, the residual volume of a fibrin sponge with PGA fiber incorporation was significant compared with that of a fibrin sponge without PGA fiber.
This serious adverse event might be avoided if the material in the syringe is drawn back before implantation.
It must also be considered that it was not common practice to implant the metal back (metaglene) in a caudal position, consequently at the time of implantation, as recommended today (Nyffeler et al. 2005).
And even in written agreements, the court found, either party has the right to back out before implantation occurs.
