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They also convincingly show that WWP-1 is involved in DR by using a method that utilizes bacterial dilution to impart lifespan extension.
This raises the question of whether other drugs (such as methotrexate, an inhibitor of dihydrofolate reductase and widely used in chemotherapy) might also impart lifespan extension effects when added to the worm diet.
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In this view, the decline in peroxidation index (PI) with increasing lifespan would impart survival benefits by reducing the principal substrates of lipoperoxidation, a process responsible for the only free-radical chain reactions of biological significance [ 32].
This suggests that most of the lifespan extension imparted by the pma1-105 allele requires V-ATPase function, but that the mechanism of lifespan extension is not limited to increased vacuolar acidification.
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The lifespan extension for animals imparted by the sirtuins is up to 50% [ 3].
Similarly, the mechanism by which dTSPO depletion imparts protection from neurodegenerative disease and extension of lifespan in the face of OXPHOS dysfunction merits further investigation.
Given the common view that DAF-16 is a master regulator of longevity, this would implicate that the metabolic changes imparted by daf-2 e1370) daf-2 e1370ry, rareer thauxiliaryial, mechanisms of lifespan extension.
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