Remarkably, adult laboratory mice exhibit limited susceptibility to USUV infection, which has impaired the analysis of the immune responses, thus complicating the evaluation of virus host interactions and of vaccine candidates against this pathogen.
Taken together, as hepatic Sepp, Pstk and SecS mRNA expression were not impaired, the analysis of hepatic gene expression failed to explain the decreased serum Se concentrations in TRα1+m mice and its increase upon T3 treatment.
Endogenous chemokine expression could thus impair the analysis of exogenous chemokine transport across the blood brain barrier in vitro.
Confounding conditions that may impair the analysis in subjects with hypertensive retinopathy, such as microaneurysms, exudates, and hemorrhages, were removed by using the Remove Outliers filter enclosed in Image-J and by subtracting the resulting mask from the retinal image.
Depending on the conditions of fixation and storage, the extracted DNA is more or less fragmented, which may have impaired the analysis of mutations in the APC gene more than in the K-ras gene, since the analysis of the latter is based on the amplification of a smaller gene fragment.
We thus applied the PAT-ChIP procedure to LMD lung tumor samples originating from the K-rasv12 transgenic mouse model using six different antibodies; notably, the LMD procedure did not impair the analysis by ChIP of the extracted chromatin, even when studying very small amounts and after histological staining.
As we will see below, this problem does not overly impair the analysis of host specificity in Lecanicillium spp. The Lecanicillium spp. group includes a number of important species that are used for the control of pests and diseases in agriculture.
Extirpation was not shown to have an affect on DPD enzyme activity in CRC samples (Sadahiro et al, 2003); however, delays in fixation following excision have been shown to impair the analysis of the oestrogen receptor (ER) status in breast cancer patients (Von Wasielewski et al, 1998).
To exclude that this species difference impairs the expression analysis, a sequence comparison was performed using the current version (release 16) of the Sanger miRBase (see Table S2).
Bouin fixation should be avoided since it may impair the molecular analysis feasibility.
